Figure 5.

Regulations of growth and metabolism in transgenic mice. (a) Mice breeding atlas. (b) Sequence alignment of skeletal muscle-specific miR-146a-5p knockout (mKO) and Flox. (c) The expression of miR-146a-5p in GAS and TA of mKO mice was detected by quantitative PCR (n = 4). (d) Body weight change curve of Flox and mKO mice fed a high-fat diet (n = 4). (e) The muscle weight for GAS and TA of Flox and mKO mice fed a high-fat diet (n = 4). (f) Accumulate feed intake of Flox and mKO fed HFD (n = 4). (g) IPITT blood glucose changes in Flox and mKO mice (n = 4). (h) IPGTT blood glucose changes in Flox and mKO mice (n = 4). (i,j) Oxygen consumption (n = 4). (k,l) CO₂ release (n = 4). Values are presented as means ± SEM, * p < 0.05, and ** p < 0.01, according to the non-paired Student’s t-test or one-way ANOVA between individual groups.