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. 2023 Feb 25;24(5):4561. doi: 10.3390/ijms24054561

Figure 6.

Figure 6

Effects of skeletal muscle-specific miR-146a-5p knockout on adipogenesis in mice. (a) Body composition of Flox and mKO mice. (b) Body imaging of Flox and mKO mice. (c) Tissue weight (IngWAT and EpiWAT) of Flox and mKO mice after feeding with a high-fat diet (HFD) (n = 4). (d) HE staining image of IngWAT and EpiWAT in Flox and mKO mice fed with HFD (scale bar = 50 μm). (e) Fluorescence quantitative PCR detection of adipogenesis-related genes GDF5, PPARγ, C/EBPα, and fatty acid synthesis-related genes CD36, FABP4, FASN in IngWAT of Flox and mKO mice (n = 4). (f,g) Western Blot detection of adipogenesis-related proteins GDF5, PPARγ, C/EBPα, and fatty acid synthesis-related proteins CD36, FABP4, and FASN in IngWAT of Flox and mKO mice (n = 4). (h) Fluorescence quantitative PCR detection of adipogenesis-related genes GDF5, PPARγ, C/EBPα, and fatty acid synthesis-related genes CD36, FABP4, FASN in EpiWAT of Flox and mKO mice (n = 4). (i,j) Western Blot detection of adipogenesis-related proteins GDF5, PPARγ, C/EBPα, and fatty acid synthesis-related proteins CD36, FABP4, FASN in EpiWAT of Flox and mKO mice (n = 4). Values are presented as means ± SEM, * p < 0.05, and ** p < 0.01, according to the non-paired Student’s t-test or one-way ANOVA between individual groups.