Figure 1.
SBPH nymph infestation contributes to SBPH feeding and survival on rice. All of the choice and no choice assays for SBPH were performed after 48 h of continuous nymph pre-infestation; untreated SBPH was used as the control. A and B, SBPH host preference in choice tests. The mean number + SE (n = 15) of fourth-instar SBPH nymphs (A) and female adults (B) per plant for plant pairs (SBPH nymphs pre-infested rice vs. control). n, number of biological replicates. Fifteen fourth-instar nymphs or female adults were released per replicate, and the experiment was repeated 12 times. Statistical significance was calculated using GraphPad Prism 8 (Version 8.0.2, GraphPad Software, La Jolla, CA, USA). ** p < 0.01, Student’s t-tests. (C) Mean number of eggs laid by five female adults that fed on the control or SBPH nymph pre-infested rice plants for two days; the experiment was repeated 15 times. ** p < 0.01, Student’s t-tests. The model figures in the right half of the graph depict the SBPH choice and no choice assays.