Figure 2.

aPTT tests—Coagulation assay (aPTT) performed in vitro with human plasma in a Dade Behring semiautomatic coagulometer. PPP was incubated with pDrTI (A) or pDrTI-RI (B) in the presence of cephalin. After incubation, 0.02% CaCl₂ was added and the time for fibrin clot formation was recorded. (C) Coagulation assay ex vivo. Animals were pre-treated by retro-orbital injection with the substances 30 min prior to euthanasia. Whole blood was collected by cardiac puncture with 3.8% sodium citrate, 1:9, and centrifuged to obtain PPP. Then, a coagulation assay was performed in a Dade Behring semiautomatic coagulometer. R = Ratio between the clotting time of the compounds under study and the control. Assays were performed in triplicate. Statistical analysis was made using t-test with Welch’s correction using GraphPad Prism 8. n.s.: non-significant; * p < 0.05; ** p < 0.01; *** p < 0.001.