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. 2023 Feb 23;12(5):1810. doi: 10.3390/jcm12051810

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Platelet Aggregation—PRP was obtained from healthy individuals. Different concentrations of the peptides were added to the PRP and incubated at 37 °C for 5 min. After the incubation time, the agonist (ADP, arachidonic acid, or collagen I) was added and the reaction was monitored in an automatic aggregometer Chrono-log 700 through transmittance/impedance. Assays were performed in triplicate. Results are shown as percentage aggregation, ADP induced aggregation was inhibited by pDrTI (A) and pDrTI-RI (B); Arachidonic acid induced aggregation was inhibited by pDrTI (C) and pDrTI-RI (D) and collagen I induced aggregation was not inhibited (E). Statistical analysis was made using one-way ANOVA with Welch’s correction using GraphPad Prism 8. * p < 0.05; ** p < 0.01; *** p < 0.001.