Figure 5.

Platelet behavior after treatment with DrTI and Aspirin—(A) DrTI (4 mg/kg), Aspirin (30 mg/kg), and NaCl (0.9%) were administered by retro-orbital injection and aspirin by gavage 30 min before dissection. Rhodamine 6G was administered by retro-orbital injection to label mitochondria in platelets and leukocytes. Arterial injury was induced by the application of filter paper saturated with 15% FeCl₃. The injured artery was monitored for 12 min with an image capture every 3 min using an intravital fluorescence microscope. (B) Mean fluorescence was quantified using the software ImageJ version 1.46r. N = 3 animals.