Figure 5.

ICD reduces the activation of LPS-induced inflammation-related signaling pathway in bone marrow-derived macrophages. (A,B) Western blot analysis activation of NF-kB and MAPK signaling pathway in macrophages stimulated with LPS at different time points as indicated, pretreated with ICD or not. The ratios of p-p65/p65 (C) and p-JNK/JNK (D) were quantified with ImageJ software (v1.53). The mean and SD of at least three independent experiments are shown. * p < 0.05, ** p < 0.01 indicate significant differences between groups, as determined by a two-tailed paired Student’s t-test.