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. 2023 Feb 21;28(5):2005. doi: 10.3390/molecules28052005

Figure 1.

Figure 1

Anti-inflammatory effects of BBD on LPS-induced zebrafish inflammation model. (A) Flow chart of LPS-induced inflammation modeling. (B) Representative photos of zebrafish larvae of different degrees of inflammation. Embryos were classified as normal (no macrophage aggregation), mild (≤30 macrophages in yolk), medium (>30 macrophages but without an aggregation cluster), or severe (>30 macrophages and with an aggregation cluster). The red dotted line indicates zebrafish yolks, the yellow arrows indicate macrophages, and the red arrow indicates the aggregation cluster. (C) Toxicity of BBD treatment (n = 10). Larvae with cardiac edema, or body distortion, are defined as unhealthy. (D) The classification of inflammatory phenotypes in zebrafish embryos of different groups (n > 10). Control: PBS injection; model: LPS injection; DEX was used as positive control. The combined percentage of the medium and severe phenotypes was statistically analyzed among different groups. (E) The mRNA levels of IL-6, TNF-α, and CCL-2 in zebrafish embryos of different groups (n > 10). All the data are presented as the mean ± SEM of three independent experiments. # Compared with the control group; * compared with the model group; *, p < 0.05, ## or **, p < 0.01.