FIG. 2.

Adherence efficiency of pO157-cured mutants (O157Cu). Seven clones of O157Cu were identified as 11, 12, 15, 18, 27, 28, and 35. The number of MC on Caco-2 cells infected by bacteria and visualized as described in Fig. 1 was scored as the sum of 20 microscopic fields. Independent experiments were done at least 33 times with clone 11, 18 times with clone 12, 21 times with clone 15, 24 times with clone 18, 27 times with clone 27, 30 times with clone 28, 27 times with clone 35, and 18 times with clone 38. The data shown are the means and standard deviations of three representative experiments. The experiments were performed on separate days; therefore, each value was normalized to that of the wild type (WT; 100%).