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. 2023 Feb 21;16(5):1765. doi: 10.3390/ma16051765

Figure 2.

Figure 2

Methodology used. (A) Immersion of specimens in culture plates in a medium containing BHI + inoculum + 10% sucrose, for 8 h; (B) transfer of specimens to a new medium containing BHI + glucose every night; (C) sucrose baths: specimens washed with 0.01% NaCl and immersed in medium (BHI + 10% glucose) for 3 min, washed again in NaCl, and returned to the initial plate (8 times a day for 5 days); (D) sonication of specimens in an Eppendorf tube of 0.001% NaCl; (E) serial dilution of Eppendorf 0; and (F) centrifugation of Eppendorf 0, dilution of Eppendorf 0, and plating for CFU counting.