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Recombinant phage PHB20. (A) Construction of donor plasmids. All phage-tail fiber gene templates were performed using the CloneExpress® II One Step Cloning Kit. (B) Schematic overview of the workflow of PHB20 genome editing. (C) Sanger sequencing of the target genes. The numbers 1−14 represent recombinant phages. (D) Plaquing assay with WT−PHB20 and engineered phage PHB20(P6). Ten-fold serial dilutions of phage lysates were spotted on bacterial lawns and incubated at 37 °C for 6 h.
