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(A) The activity of compound 9j against four kinases (EGFR, ALK, EGFR L858R T790M, and EML4-ALK). (B) H1975 and H2228 cells were treated with 9j for 2–4 h; the staining for phosphorylated EGFR (p-EGFR) and phosphorylated ALK (p-ALK) was shown as red fluorescence. (C) WB analysis of phosphorylated proteins in drug-treated H1975 and H2228 cells.
