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TGF-β activity following rBCG infection of THP-1 cells. TGF-β activity was measured using the Mv1Lu assay and supernatants prepared from THP-1 cells infected for 16 h with 3a/LAP, 4a/LAP, or vector control BCG at a multiplicity of infection of 10 (solid bars), 1 (hatched bars), or 0.1 (open bars) bacterium/cell. A significant reduction in TGF-β activity was observed with each of the LAP-BCG constructs (P < 0.05). The standard error is shown for triplicate samples.
