Skip to main content
. Author manuscript; available in PMC: 2023 Mar 10.
Published in final edited form as: Nat Struct Mol Biol. 2022 Dec 19;30(1):31–37. doi: 10.1038/s41594-022-00886-5

Extended Data Figure 6: cBAF readily remodels extended nucleosome arrays but requires PU.1 to access H1-compacted arrays.

Extended Data Figure 6:

a, silver-stain of affinity-purified cBAF complexes from mammalian HEK293T cells expressing HA-DPF2. b, Schematic of XbaI accessibility assay with extended arrays and cBAF remodeling complex. c, Agarose gel visualization of XbaI accessibility assay of extended nucleosome arrays (no linker histone) incubated without and with cBAF plus ATP. d, Schematic of DNase I digestion analysis of transcription factors and cBAF with H1-compacted arrays. e, DNase I digestion analysis H1-compacted nucleosome arrays incubated alone (no TF, lanes 2 and 5), PU.1 alone (lanes 3 and 6), and PU.1 with cBAF and ATP (lanes 4 and 7). The same samples were used to perform nanopore sequencing shown in Fig. 3b.