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. Author manuscript; available in PMC: 2023 Mar 10.
Published in final edited form as: Nat Struct Mol Biol. 2022 Dec 19;30(1):31–37. doi: 10.1038/s41594-022-00886-5

Extended Data Figure 7: Hierarchy of chromatin binding by PU.1 WT and mutants.

Extended Data Figure 7:

a-c, Representative EMSAs showing the affinity of increasing amounts of a, WT PU.1, b, ΔIDR, c, ΔAQ to Cy5-labeled Cx3cr1 free DNA (black arrows) or mononucleosomes (white arrows). d, Schematic of EMSA performed with transcription factors and extended nucleosome arrays e, EMSA showing the affinity of increasing amounts of WT PU.1 (lanes 1–6), ΔIDR (lanes 7–12), and ΔAQ (lanes 13–18) to Cy5-labeled extended WT or TF motif mutant Cx3cr1 nucleosome arrays. f, Schematic of EMSA performed with transcription factors and H1-compacted nucleosome arrays. g, Representative EMSA showing the affinity of increasing amounts of WT PU.1 (lanes 1–6), ΔIDR (lanes 7–12), and ΔAQ (lanes 13–18) to Cy5-labeled H1-compacted WT or TF motif mutant Cx3cr1 nucleosome arrays.