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. Author manuscript; available in PMC: 2023 Mar 10.
Published in final edited form as: Nat Struct Mol Biol. 2022 Dec 19;30(1):31–37. doi: 10.1038/s41594-022-00886-5

Figure 2: Domains outside of DBDs are needed to open chromatin.

Figure 2:

a, Illustration of wild-type (WT) and DNA-binding domain (DBD) recombinant PU.1 and C/EBPα. b, Coomassie stained SDS-PAGE of 1 ug of each indicated purified protein. c, DNase I digestion analysis of WT and DBD TF opening of H1-compacted chromatin. d, Quantified Cy5 signal in each lane normalized to no TF control (n=4, *p < 0.05). Data are mean ± SD and significance was determined by Student’s unpaired t-test.