. 2023 Mar 10;18(3):e0280688. doi: 10.1371/journal.pone.0280688

Table 2. NDV Fusion (F) gene tiled fragments and designed PCR primers.

	Primer	Fragment Position	Sequence (5’ to 3’)	Amplicon(bp)
Primer mix	NDVF_1561F	Fragment 1 (95–546)	`TTGATGGCAGGCCTCTTGC`	497
	NDVF_IR2-2	Fragment 1 (95–546)	`CATCTTCCCAACTGCCACTG`	497
	NDVF_IF2	Fragment 2 (469–1007)	`AGCATTGCTGCAACCAATGAGGC`	538
	NDVF_IR1	Fragment 2 (469–1007)	`GAGGTGTCAAGTTCTTCTATCAC`	538
	NDVF_IF1	Fragment 3 (882–1398)	`CCTAAATAATATGCGTGCCAC`	518
	NDVF_IR3	Fragment 3 (882–1398)	`CTCAGTTGAKATATCRAGATTGCCTG`	518
	NDVF_IF3-3	Fragment 4 (1287–1655)	`AGACGGGATAACTCTGAGGCT`	369
	NDVF_1561R	Fragment 4 (1287–1655)	`TTTGTAGTGGCTCTCATCTG`	369