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. 2023 Mar 10;18(3):e0280688. doi: 10.1371/journal.pone.0280688

Table 6. Biosecurity practices in backyard farms(n = 36) of Nepal along with antibody ELISA results for NDV and IAV.

ELISA results provide an insight into any possible correlations between biosecurity measures and presence of diseases.

Districts Chicken Breed Biosecurity Measures Antibody ELISA
(4 farms per district) Broilers Breeds At least one Disinfectant Used At least one PPE Used Vaccines Used ELISA_NDV ELISA_IAV
Cobb 500 Giriraj Kadaknath Kuroiler Sakhini Pwakh Ulte Yes No Yes No Yes No Positive Negative Positive Negative
Bhaktapur 1 2 0 1 0 0 0 4 0 4 0 4 1 3 0 4
Chitwan 0 1 0 1 1 1 1 3 1 3 0 4 0 4 0 4
Dang 0 3 1 0 0 0 2 2 1 3 0 4 2 2 0 4
Jhapa* NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA
Kailali 1 0 1 0 1 1 0 4 0 4 0 4 3 1 1 3
Kathmandu 1 3 0 0 0 0 2 2 0 4 0 4 0 4 0 4
Lalitpur 0 0 0 2 1 1 2 2 0 4 0 4 0 4 0 4
Morang 0 2 1 0 0 1 1 3 2 2 0 4 0 4 0 4
Nawalparasi 0 0 0 3 1 0 2 2 1 3 1 3 0 4 1 3
Sunsari 0 0 0 2 1 1 3 1 1 3 0 4 1 3 1 3
Percentage 7.5 27.5 7.5 22.5 12.5 12.5 32.5 57.5 15 75 2.5 87.5 17.5 72.5 7.5 82.5

*Farms in Jhapa did not consent to sampling and are thus listed as NA. Total farms questioned are n = 36.