Table 3. Details of markers used for molecular screening of blast resistance genes in 52 rice accessions.

Genes	Markers	Forward (5’ - 3’)	Reverse (5’ - 3’)	Type of Marker*	Annealing Temperature (°C)	References
Pit	tk59-1	ATGATAACCTCATCCTCAATAAGT	GTTGGAGCTACGGTTGTTCAG	FM	54	[48]
Pid1(t)	RM262	CATTCCGTCTCGGCTCAACT	CAGAGCAAGGTGGCTTGC	LM	55	[63]
Pish	RM6648	GATCGATCATGGCCAGAGAG	ACAGCAGGTTGATGAGGACC	LM	55	[34]
Pb1	RM26998	ACGCACGCACATCCTCTTCC	CGGTTCTCCATCTGAAATCCCTAGC	LM	55	[21]
Pi33	RM72	CCGGCGATAAAACAATGAG	GCATCGGTCCTAACTAAGGG	LM	55	[64]
Pikhahe-1(t)	RM17496	TAAACGGTGTGCAGCTTCTG	TATTATGGGCGGTCGCTAAC	LM	54	[65]
pi21	pi21-79-3	GATCCTCATCGTCGACGTCTGGC	AGGGTACGGCACCAGCTTG	InDel	55	[27]
Pi56	CRG4-2	CCTGTCAGTCTTTCCGAGAG	GAATCCGGTAGCTCAAGGTG	Gene-specific	55	[66]
Pi65	SNP_3	TGCCACCAGCCATCTTCAACAT	ACCACATCACTCATCGCCATCC	InDel	54	[71]
Pi36	RM5647	ACTCCGACTGCAGTTTTTGC	AACTTGGTCGTGGACAGTGC	LM	55	[72]
Pi49	RM6094	TGCTTGATCTGTGTTCGTCC	TAGCAGCACCAGCATGAAAG	LM	55	[67]
Pi48	RM5364	GTATTACGCTCGATAGCGGC	GTATCCTTTCTCGCAATCGC	LM	55	[68]
Pib	Pb28	GACTCGGTCGACCAATTCGCC	ATCAGGCCAGGCCAGATTTG	SNP	60	[48]
Piz	Z56592	GGACCCGCGTTTTCCACGTGTAA	AGGAATCTATTGCTAAGCATGAC	SNP	60	[48]
Piz-t	Zt56591	TTGCTGAGCCATTGTTAAACA	ATCTCTTCATATATATGAAGGCCAC	SNP	60	[48]
Pik	K39512	GCCACATCAATGGCTACAACGTT	CCAGAATTTACAGGCTCTGG	SNP	60	[48]
Pik-p	K3957	ATAGTTGAATGTATGGAATGGAAT	CTGCGCCAAGCAATAAAGTC	SNP	60	[48]
Pik-h	Candidate gene marker	CATGAGTTCCATTTACTATTCCTC	ACATTGGTAGTAGTGCAATGTCA	Gene-based marker	55	[69]
Pi9	Pi9-i	GCTGTGCTCCAAATGAGGAT	GCGATCTCACATCCTTTGCT	FNP	54	[52]
Pi2	Pi2-i	CAGCGATGGTATGAGCACAA	CGTTCCTATACTGCCACATCG	FNP	52	[52]
Pita/Pita2	Pita3	AGTCGTGCGATGCGAGGACAGAAAC	GCATTCTCCAACCCTTTTGCATGCAT	SNP	59	[48]
Pi1	RM1233	GTGTAAATCATGGGCACGTG	AGATTGGCTCCTGAAGAAGG	SSR	55	[40]
Pi5	40N23R	TGTGAGGCAACAATGCCTATTGCG	CTATGAGTTCACTATGTGGAGGCT	InDel	55	[40]
Pikm	k2167	CGTGCTGTCGCCTGAATCTG	CACGAACAAGAGTGTGTCGG	InDel	55	[40]
Pi25	CAP1	TGAAATGGGTGAAAGATGAG	GCCACATCATAATTCCTTGA	CAPS	55	[70]

* FM, functional marker; LM, linked marker; InDel, insertion-deletion marker; FNP, functional nucleotide polymorphism; SNP, single nucleotide polymorphism; CAPS, Cleaved Amplified Polymorphism Sequences