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HasAyp is secreted through the S. marcescens Has system. Samples were prepared as described in Materials and Methods and Coomassie blue stained after SDS-PAGE analysis. The equivalent of 1 ml of supernatants from cultures at an OD600 of 0.5 was loaded in each lane. Lanes: 1, molecular size markers; 2, MC4100 hemA; 3, MC4100 hemA(pSyc150); 4, MC4100 hemA(pHasAyp); 5, MC4100 hemA(pSyc150, pHasAyp); 6, MC4100 hemA(pSyc150, pHasAsm); 7, molecular size markers.
