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Growth of Y. pestis KIM6 (has+ hmu+ ), KIM6–2061.1 (has+ hmu mutant), and KIM6–2085 (has hmu double mutant) cells at 28°C in deferrated PMH2 with hemoglobin as the sole iron source. Iron-depleted cells were transferred to PMH2 containing 50 μM EDDA alone (+) or with hemoglobin at concentrations of 1.25 (▵), 2.5 (▴), 5 (○), or 10 (●) μM. These graphs depict one of two separate experiments with similar results.
