TABLE 3.
β-Galactosidase activities of Y. pestis strains containing lacZ reporters
| Straina | β-Galactosidase activity (mean ± SE Miller units)a
|
−Fe/+Fe ratio | |
|---|---|---|---|
| +Fe | −Fe | ||
| KIM6(pEU730)+ | 118 ± 14 | 203 ± 71 | 1.7 |
| KIM6(pEUHas)+ | 8,264 ± 1,532 | 39,738 ± 6,182 | 4.8 |
| KIM6(pEU730) | 227 ± 49 | 285 ± 26 | 1.3 |
| KIM6(pEUHas) | 5,822 ± 1,208 | 19,023 ± 6,597 | 3.3 |
| KIM6-2030(pEU730) | 200 ± 46 | 446 ± 62 | 2.2 |
| KIM6-2030(pEUHas) | 22,152 ± 4,492 | 28,674 ± 5,235 | 1.3 |
a
Bacterial cells were grown in deferrated PMH2 at 37°C and harvested during mid-log-phase growth. Whole-cell lysates were assayed for β-galactosidase activity as described in reference 28.