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. 2023 Mar 3;13(5):929. doi: 10.3390/nano13050929

Figure 5.

Figure 5

In vitro irritation test. (A) The 2D skin irritation test on JB6 cell culture. The cells were cultured in 96-well plates and treated with T-RCAR-3, free drug or PT for 48 h. MTT assay was used to evaluate the cell viability. The data were normalized by controls without treatment (n = 3). Only in treatment at the highest concentrations was there a statistically significant reduction in cell viability (ANOVA). ****: p < 0.0001. (B) The 3D skin irritation test on EpiDerm. MTT assay was conducted on EPI-200 tissues after topical applications of negative control (sterile DPBS), positive control (strong irritant, 5% SDS) or test agents in various doses for 60 min. The data were normalized by negative controls (n = 3). Only the positive controls (5% SDS) showed statistically significant reduction in cell viability (ANOVA). ****: p < 0.0001. ns: not significant.