Table 1.
Target Gene Symbol | Forward Primer Sequences (5′→3′) | Reverse Primer Sequence (5′→3′) | Amplicon Length (bp) |
---|---|---|---|
ND1 (mitochondrially encoded NADH: ubiquinone oxidoreductase core subunit 1) | Long fragment: ATGGCCAACCTCCTACTCCT | Long fragment: GATGAGTGTGCCTGCAAAGA | 1214 |
Small fragment: CCTAAAACCCGCCACATCTA | Small fragment: GCCTAGGTTGAGGTTGACCA | 124 | |
ND5 (mitochondrially encoded NADH: ubiquinone oxidoreductase core subunit 5) | Long fragment: TCCAACTCATGAGACCCACA | Long fragment: AGGTGATGATGGAGGTGGAG | 1156 |
Small fragment: AGGCGCTATCACCACTCTGT | Small fragment: TTGGTTGATGCCGATTGTAA | 124 |