Table 3.
Primers used for amplification of sequences.
S. No. | Oligo Name | Sequence (5`à 3`) | Tm (°C) | GC Content |
---|---|---|---|---|
1. | ITS Forward | TCCGTAGGTGAACCTGCGG | 57 | 63.15% |
2. | ITS Reverse | TCCTCCGCTTATTGATATGC | 53 | 45% |
Primers used for amplification of sequences.
S. No. | Oligo Name | Sequence (5`à 3`) | Tm (°C) | GC Content |
---|---|---|---|---|
1. | ITS Forward | TCCGTAGGTGAACCTGCGG | 57 | 63.15% |
2. | ITS Reverse | TCCTCCGCTTATTGATATGC | 53 | 45% |