FIG. 4.

Cytoskeletal rearrangement and cell distribution according to DNA content of HeLa cells 72 h after exposition to E22 in the presence or absence of the Rho inhibitor DC3B. HeLa cells were pretreated for 2 h with DC3B, and then E22 was added and the interaction was continued for 4 h. Control cells were treated with DC3B but left uninfected. After several washes, the cells were incubated for 72 h without bacteria and toxin. F-actin was stained with rhodamine-phalloidin, and vinculin was labeled by FITC indirect immunofluorescence. Corresponding cell distribution according to DNA content was determined by flow cytometry.