Figure 5.

Effect of 300 pg/mouse AT-RvD1 on the increase of MMP-9 activity and related collagen damage in hairless mouse skin exposed to 4.14 J/cm² UVB radiation. Skin samples were collected 12 h after the exposure. Representative image of acrylamide-SDS-gelatin zymography gel showing discolored zones that represent MMP-9 activity (A); MMP-9 activity per group (B); collagen fiber intensity evaluated as a percentage of staining, compared per group (C); and representative image of collagen fiber intensity and bundles, shown in blue, of the three tested groups (D). Images in panel (D) were analyzed and quantitated using the Image J software, version 1.51 (U. S. National Institutes of Health, Bethesda, MD, USA) to generate the results shown in panel (C). Bars represent means ± SEM. Panel (B) presents the results of four pools of the dorsal skin of two mice per pool. For panels (C,D), two separate experiments with five groups of 5 mice per group per experiment were performed. Statistical analysis was performed by one-way ANOVA, followed by Tukey´s post hoc test. * p < 0.05 compared to the nonradiated control group (orange bars); # p < 0.05 compared to the radiated control group (purple bars).