Table 2.
Effect of combinations of 25 µM 2,4-D and cytokinins on SE in C. orbiculata.
Cytokinin Type | Cytokinin Conc. (µM) | SoE Induction (%) | Number of SoEs per Explant | ||
---|---|---|---|---|---|
Control (25 µM 2,4-D) | 0 | 60.6 ± 3.5 h | 14.9 ± 2.1 gf | ||
6-BA | 1.2 | 66.6 ± 2.7 g | 11.8 ± 1.4 g | ||
2.2 | 74.8 ± 3.0 ef | 18.1 ± 2.9 e | |||
4.4 | 88.7 ± 4.6 c | 21.3 ± 2.7 d | |||
8.8 | 52.6 ± 5.8 j | 7.4 ± 1.2 h | |||
KN | 1.2 | 72.0 ± 3.9 f | 15.9 ± 2.2 f | ||
2.2 | 79.3 ± 3.1 d | 22.6 ± 1.8 d | |||
4.4 | 92.3 ± 3.0 b | 27.0 ± 2.9 c | |||
8.8 | 56.9 ± 3.1 i | 8.0 ± 2.2 h | |||
TDZ | 1.2 | 90.6 ± 2.7 bc | 29.3 ± 2.9 b | ||
2.2 | 97.2 ± 2.8 a | 35.8 ± 2.5 a | |||
4.4 | 76.9 ± 4.3 de | 20.8 ± 1.9 d | |||
8.8 | 44.6 ± 3.4 k | 13.6 ± 2.2 g | |||
ANOVA | R-square | 0.9561 | 0.9356 | ||
Coefficient of variation | 4.89 | 11.91 | |||
Root mean square error | 3.64 | 2.29 | |||
F-value | p-value | F-value | p-value | ||
Cytokinin type | 31.38 | 0.001 | 181.54 | 0.001 | |
Cytokinin conc. | 513.87 | 0.001 | 247.16 | 0.001 | |
Cytokinin type * Cytokinin conc. | 80.74 | 0.001 | 48.55 | 0.001 |
Means ± SDs within columns (3 and 4) followed by different alphabets (a–k) are significantly different according to DMRT at p < 0.05. *—Interaction.