Table 2.
Rodent diet with 10 or 60 kcal% fat and modification to fat sources and fat level. * The control diet was not investigated in this study and is included for reference purposes only. ** Vitamin mix (V1001): vitamin A acetate (500,000 IU/g), vitamin D3 (100,000 IU/g), vitamin E acetate (500 IU/g), menadione sodium bisulfate (62.5% menadione), biotin (1.0%), cyanocobalamin (0.1%), folic acid (0.2 g), nicotinic acid (3 g), calcium pantothenate (1.6 g), pyridoxine-HCl (0.7 g), riboflavin (0.6 g), thiamine HCl (0.6 g) and sucrose (978.42 g).
| * Control Diet | HFD 50:50 | |||
|---|---|---|---|---|
| g | kcal | g | kcal | |
| Protein (% by weight) | 19.2 | 20 | 26.2 | 20 |
| Carbohydrate (% by weight) | 67.3 | 70 | 26.3 | 20 |
| Fat (% by weight) | 4.3 | 10 | 34.9 | 60 |
| Total kcal | 100 | 100 | ||
| kcal/g | 3.85 | 5.24 | ||
| Ingredients | ||||
| Protein Casein, 80 Mesh |
200 | 800 | 200 | 800 |
| L-Cystine | 3 | 12 | 3 | 12 |
| Carbohydrate Corn Starch Maltodextrin 10 Sucrose Cellulose, BW200 |
500 100 100 50 |
2000 400 400 0 |
0 125 68.8 50 |
0 500 275 0 |
| Lipid Soybean Oil Lard CoconutOil, Hydrogenated Safflower Oil Cocoa Butter |
10 5 0 0 30 |
90 45 0 0 270 |
25 245 0 0 0 |
225 2205 0 0 0 |
| Mineral Mix S10026 Dicalcium phosphate Calcium carbonate Potassium citrate, 1 H2O |
13 5.5 16.5 |
0 0 0 |
13 5.5 16.5 |
0 0 0 |
| Vitamin Mix V10001 ** Choline Bitartrate |
10 2 |
40 0 |
10 2 |
40 0 |
| FD&C Yellow Dye #5 FD&C Red Dye #40 FD&C Blue Dye #1 |
0 0 0 |
0 0 0 |
0 0 0.05 |
0 0 0 |
| Total | 1055 | 4057 | 773.85 | 4057 |