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. 2023 Mar 3;28(5):2358. doi: 10.3390/molecules28052358

Figure 2.

Figure 2

The growth inhibitory activity and stability of DHA and UDCMe-Z-DHA in HCC cells. (A) The effect of DHA and UDCMe-Z-DHA on the growth of HepG2 and Huh-7 HCC cells. Cells were treated for 72 h. HepG2 cells were significantly more sensitive to DHA and UDCMe-Z-DHA than Huh-7 cells. (B) Time-course study of DHA and UDCMe-Z-DHA in HepG2 cells. Cells were treated for 24–72 h. (C) The effect of daily change of DHA and UDCMe-Z-DHA on growth of HepG2 cells. Cells were treated for 72 h with no change or daily change of medium with fresh compounds. (D) Stability of DHA and UDCMe-Z-DHA in culture medium. Compounds were preincubated in medium for the indicated time periods at 37 °C before added to Hep2 cells for 72 h. The cell viability was measured by the MTT assay. (E) Chemical stability of UDCMe-Z-DHA in culture medium measured by HPLC-MS/MS. Data are presented as mean ± SEM of at least three independent experiments. Statistical significance was assessed by two-tailed Student’s t-test. * p < 0.05, ** p < 0.01, *** p < 0.001.