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. 2023 Mar 3;28(5):2358. doi: 10.3390/molecules28052358

Figure 4.

Figure 4

DHA and UDCMe-Z-DHA induce ROS in HepG2 cells. (A) Time course of ROS induction by DHA and UDCMe-Z-DHA. (B) NAC reversed ROS generation induced by DHA but not UDCMe-Z-DHA. (C) NAC reversed the growth inhibitory effect of DHA. (D) NAC did not affect the growth inhibitory effect of UDCMe-Z-DHA. In (C,D), HepG2 cells were treated with DHA or UDCMe-Z-DHA in the absence or presence of 2 mM NAC for 72 h, and cell viability was measured by the MTT assay. Data are presented as mean ± SEM of at least three independent experiments. Statistical significance was assessed by two-tailed Student’s t-test. * p < 0.05, ** p < 0.01.