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. 2023 Mar 3;28(5):2358. doi: 10.3390/molecules28052358

Figure 5.

Figure 5

DHA and UDCMe-Z-DHA induced MMP loss in HCC cells. (A) HepG2 cells. (B) Huh-7 cells. HCC cells were exposed to indicated concentrations of DHA and UDCMe-Z-DHA for 48 h, and 5 μg/mL JC-1 was added to the cells 30 min before the termination of the incubation period at 37 °C. Cells were then harvested for flow cytometric analysis of JC-1 fluorescence. The percentages of cells with depolarization of MMP (labeled in green) were analyzed by CellQuest software. Data are presented as mean ± SEM of at least three independent experiments. Statistical significance was assessed by two-tailed Student’s t-test. * p < 0.05, ** p < 0.01, *** p < 0.001.