Skip to main content
. 2023 Feb 17;12:e82579. doi: 10.7554/eLife.82579

Figure 3. BHC module has both kinetochore-dependent and -independent functions in spindle assembly and chromosome segregation in oocytes.

(A) Schematic of the meiotic spindle during meiosis I division (top) and stills from live imaging of meiosis I in indicated conditions (bottom). Microtubules (GFP::TBA-2α -tubulin) in green, chromosomes (mCherry::HIS-11H2B) in magenta. Time in seconds relative to anaphase I onset. Scale bar 5 µm. Graphs on the right show quantifications of meiotic defects. (B) Plots of spindle density (corrected GFP intensity, left) and spindle area (right) 45 seconds before anaphase I onset. Tests, One-way ANOVA multiple comparisons alpha = 0.05, *p<0.05, **p<0.01, ***p<0,001, ****p<0,0001, n.s. not significant. Error bars, Mean and standard deviation.

Figure 3—source data 1. Panel A source data.
Quantifications of the meiotic defects observed in indicated conditions.
Figure 3—source data 2. Panel B source data.
Quantifications of spindle density and spindle size during meiosis I in indicated conditions. Raw quantifications and corrected intensities of GFP::TBA-2α-tubulin signal (GFP Integrated Intensity/Background Noise). Details of statistical analyses are provided.

Figure 3.

Figure 3—figure supplement 1. SAC activation is not responsible for defects observed upon BHC module perturbation.

Figure 3—figure supplement 1.

(A–B) Stills from live imaging in indicated conditions. Microtubules (GFP::TBA-2α-tubulin or GFP::TBB-2β-tubulin) in green, chromosomes (mCherry::HIS-11H2B) in magenta. Time in seconds relative to anaphase I onset. Right panels indicate quantifications of meiotic defects as referred to in color key. Scale bars, 5 µm. (C) Box plot of time between anaphase I and II onsets in indicated conditions upon depletion of the spindle assembly checkpoint protein mdf-2 (left). Whiskers represent 5–95 percentile, (+) signs represent the mean. Kruskal-Wallis multiple comparisons, alpha = 0.05 (black bars), Mann-Whitney test, alpha = 0.05 (color bars), *p<0,05, **p<0.01, n.s. not significant.
Figure 3—figure supplement 1—source data 1. Panel A source data.
Quantifications of the meiotic defects in indicated conditions.
Figure 3—figure supplement 1—source data 2. Panel B-C source data.
Time measurement between anaphase I and anaphase II in indicated conditions (details of statistical tests are provided), and quantifications of meiotic defects observed.