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. 2022 Dec 8;28(3):1383–1395. doi: 10.1038/s41380-022-01898-9

Fig. 2. The level of dentate TERT determines the HPA axis activity without relevance to GR and MR.

Fig. 2

Western blot showing the levels of CRF and GAPDH in the hypothalamus (a) and the concentration of CORT in the plasma (b), of 5-week-old Tert−/− and WT mice received microinjection of 1 μl of RV-TERT-EGFP or RV-EGFP into the bilateral DGs. Two months after virus injection, the samples for western blot were prepared and the concentration of CORT in the plasma was measured. n = 4–8. One-way ANOVA. The concentration of CORT (c) and ACTH (d) in the plasma, and the number of CRF cells (e) in the PVN of 5-week-old Tert−/− mice received injection of 1 μl of RV-TERT-EGFP or RV-EGFP into the bilateral DGs. n = 5. One-way ANOVA. f In another cohort of 5-week-old Tert−/− and WT mice, 1 μl of RV-TERT-EGFP or RV-EGFP was injected into the DGs. Two months after injection, mice were exposed to 1 h-restrain stress and the plasma were collected for CORT measurement at 0.5, 1, and 5 hours after the restrain stress exposure. n = 5–6. Two-way ANOVA. g Western blot showing the level of GR, MR, and GAPDH in the DG. The samples were prepared from mice in a. One-way ANOVA. h The concentration of CORT in the plasma of Tert−/− and WT mice at different time points after sexual behavior. *P < 0.05, **P < 0.01, and ***P < 0.001, ns indicates no significant difference. Error bars indicate s.e.m.