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. 2022 Dec 8;28(3):1383–1395. doi: 10.1038/s41380-022-01898-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a The mRNA levels of TERT, GR, and GAPDH in the hippocampus of newborn mice experienced prenatal stress. n = 3-4. One-way ANOVA. b The mRNA levels of TERT, GR, and GAPDH in the DG of 3-month-old offspring experienced prenatal stress. n = 4–5. One-way ANOVA. c The concentration of CORT in the plasma of 3-month-old offspring experienced prenatal stress. n = 3–6. One-way ANOVA. d CpG sites in the promotor and exons of mouse Tert gene. e Methylation levels of Chr13:73764379 and Chr13:73764526 in the DG of 3-month-old offspring experienced prenatal stress. n = 5. One-way ANOVA, Kruskal-Wallis test. f Luciferase experiments were performed in 293T cells transfected with control plasmid pTK, plasmid pTERT, or plasmid pTERT379mut. n = 4. Two-way ANOVA. Schematic design of the construction of AD-TERT526mut-GFP, and the TERT mRNA level (g) and activity (h) in the cultured hippocampal NSCs cells infected with AD-GFP, AD-TERT-GFP, or AD-TERT526mut-GFP. n = 4–6. One-way ANOVA. i The methylation level of Chr13:73764526 and the expression of TERT in the DG after selectively editing of DNA methylation of Chr13:73764379. A volume of 1 μl of LV-Tert526-SV40-DNMT3A or LV-Scramble-SV40-DNMT3A was microinjected into the DG of 5-week mice experienced prenatal stress. Two months later, the tests were performed. n = 5. One-way ANOVA. The mRNA level of TERT, GR, MR, and GAPDH in the DG (j, k) and the concentration of CORT in the plasma (l) of 3-month-old offspring infused LV-TERT-EGFP or LV-EGFP into the DG of 5 weeks old mice experienced prenatal stress. n = 4. One-way ANOVA. m, n Representative photos and analytical data showing the number of c-FOS⁺ cells in the DG of Tert^−/− and WT mice with or without prenatal stress. Acute stress (1-hour restraining) was performed 30 minutes before collection of the plasma and perfusion with 4% PFA. n = 5. Two-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001, ns indicates no significant difference. Error bars indicate s.e.m.