Fig. 2. GABAergic neurons in LS are activated in comorbid chronic pain and anxiety.

A Schematics of the data acquisition system used for Ca²⁺ imaging with fiber photometry in combination with recording behaviors tests (von Frey, OFT, and EPM). Also shown is GCaMP6s expression in LS. Scale bar, 100 μm. B, D, F, H (Left) Schematics of fiber photometry recording in freely moving mice simultaneously receiving punctate mechanical stimulation (von Frey filament, CFA: 0.16 g and CRS: 0.4 g) or entering EPM open arms. Heatmap and average Ca²⁺ transients of LS GABAergic neurons in mice receiving von Frey punctate stimulation or entering open arms. (C, E, G, I) Statistics of the average Ca²⁺ fluorescence signaling changes across mice receiving 0.16/0.4 g von Frey stimulation or time-locked to the entry into the EPM open arms. **P < 0.01, ***P < 0.001. Data are presented as the means ± SEM. For further details of statistical data analysis see Table S1.