Skip to main content
. 2023 Feb 23;12(2):286–299. doi: 10.21037/tau-22-872

Figure 2.

Figure 2

miR-93-5p as an important transmitter in exosomes secreted by BLCA cells. (A) Scanning electron microscopy images of exosomes isolated from T24 cells; scale bars: 100 nm (B) The diameter of extracellular vesicles in Figure A as determined by particle size analysis. (C) Western blotting images of exosome markers TSG101 and CD63 in T24 cells and T24 exosomes (exo). (D) The expression of miR-93-5p in exosomes secreted by BLCA cells and HUVECs. (E) The expression of miR-93-5p in exosomes secreted by T24 cells with transfection of mimics. Exosomes transfected with miR-93-5p (exo-93-5p) expressed more miR-93-5p than exosomes transfected with negative control (exo-NC). (F) Exosomes from T24 cells fused with HUVECs. DAPI and PKH67 were used to stain HUVECs and exosomes, respectively, and exosomes were found to enter HUVECs at 6 h; scale bars: 25 µm. ***, P<0.001. BLCA, bladder cancer; HUVEC, human umbilical vein endothelial cells; DAPI, 4',6-diamidino-2-phenylindole.