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. 2023 Feb 23;12(2):286–299. doi: 10.21037/tau-22-872

Figure 5.

Figure 5

Exosomal miR-93-5p targeted PTEN directly in HUVECs and BLCA cells. (A) Analysis of the function of 18 strongly related genes in GeneMANIA. (B) The expression of PTEN in HUVECs was determined by qRT-PCR, after transfected with the exosomes above (Figure 2E), exosomes transfected with miR-93-5p (exo-93-5p) and exosomes transfected with negative control (exo-NC). (C) The expression of PTEN in 5637 cells was determined by qRT-PCR. (D) Western blotting analyzed the PTEN in each group. (E, F) Quantitative analysis of PTEN protein expression with ImageJ software. (G) The predicted miR-93-5p-binding sites in the 3'UTR of PTEN mRNA. The miRNA that potentially interacts with PTEN was calculated with miRTargetLink. (H, I) Relative levels of luciferase in 5637 cells and HUVECs cotransfected with firefly luciferase reporter plasmid containing either wild-type (WT) or mutant PTEN 3'UTR and exo-93-5p. (J-L) Transwell assay in 5637 cells that were cotransfected with a PTEN-overexpression plasmid and exo-93-5p (Figure magnification: 100×). 0.1% crystal violet solution was used for cell staining in (J). ***, P<0.001. PTEN, phosphatase and tensin homolog; BLCA, bladder cancer; HUVEC, human umbilical vein endothelial cells.