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. 2023 Feb 23;12(2):286–299. doi: 10.21037/tau-22-872

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2023 Translational Andrology and Urology. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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In vivo verification for T24 exosome-derived miR-93-5p promotion of angiogenesis and tumor growth. (A) Tumor tissues excised from tumor-implanted mice (n=6), which were received intra-tumoral injection of the exosomes above (Figure 1F) respectively, exosomes transfected with miR-93-5p (exo-93-5p) and exosomes transfected with negative control (exo-NC). (B, C) Quantitative analysis of xenografted tumor weight (B) and volume (C) (n=6). (D, E) Immunohistochemical analysis (D) of the paraffin-embedded tumor tissues with a CD31 antibody (n=6; CD31 is widely used to demonstrate the existence of endothelial tissue and to assess tumor angiogenesis) and the corresponding quantification (E); scale bars: 50 µm. **, P<0.01, and ***, P<0.001.