Fig. 6.

Glycan-binding assay of wild-type and various mutant P particles with single amino acid mutations at the HBGA-binding interface of GII.6 virus. A Glycan binding of wild-type GII.6 ​P particles with a panel of oligosaccharides representing different HBGAs. B–H Glycan binding of seven mutant P particles with single amino acid mutations at the HBGA-binding interface with the same panel of oligosaccharides. The concentrations of the P particles were 20 μg/well, while the oligosaccharides for plate coating were 0.2 μg/well. Y axes indicate the binding signals in optical densities at OD₄₅₀, while the X-axes indicate different oligosaccharides.