FIG. 2.

The induction of COX-2 transcription by CNF is Rho dependent. NIH 3T3 cells were cotransfected with 1 μg of a COX-2 promoter-luciferase reporter construct and 0.1 μg of a pCDNA3 construct expressing either LacZ or C. botulinum C3 toxin. The cells were serum starved overnight and then treated with lysates from E. coli expressing CNF (CNF) or harboring pBluescript (pBS) at a final concentration of 1 μg/ml in serum-free DMEM or left untreated (Un). The cells were harvested 8 h after treatment and assayed for luciferase activity.