Fig. 1.

Arrangement of testicular cells in mice 3D testicular organoids. A The morphological structure of testicular organoids at 0–7 days post culture. Scale bar ​= ​25 ​μm. B–D Distribution of spermatogenic cells marker DDX4 and Sertoli cells marker Vimentin (B), myoid epithelial cells marker α-SMA (C) or Leydig cells marker HSD3B1 (D) in shaped testicular organoids at 5th and 7th day post culture. Nuclei were visualized with DAPI. Scale bar, 5 ​μm. E H&E staining of the testis from 6 to 8 weeks old BALB/c male mice (ST: seminiferous tubule, Spg: spermatogonia, Spc: spermatocytes; Ser: Sertoli cell; Myo: myoid epithelial cell). Scale bar, 50 ​μm.