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Mice 3D testicular organoids can support ZIKV infection and replication. A The morphological structure of testicular organoids at 1–3 dpi. Scale bar = 25 μm. B Distribution of ZIKV antigens and spermatogenic cell marker DDX4 in testicular organoids at 1–3 dpi. Nuclei were visualized with DAPI. Scale bar, 5 μm. C & D ZIKV viral load in testicular organoids (C) or organoid cultured medium (D) at 1–3 dpi was analyzed by RT-qPCR, ∗∗P < 0.01.
