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. 2023 Mar 10;8:14. doi: 10.1038/s41536-023-00289-0

Fig. 1. PA promotes M2 macrophage polarization through JAK/STAT1 pathway.

Fig. 1

a Molecular structure of the small-molecule compound PA. b Western blotting of iNOS, IL-6, CD206 and Arg-1 in DMSO- and PA-treated rat BMDMs under LPS stimulation. c Co-immunofluorescence staining for iNOS/CD68 and CD206/CD68 in unstimulated BMDMs and DMSO, PA-treated BMDMs under LPS stimulation. Scale bar, 50 μm. d Semi-quantification of c. e Western blotting of total JAK1, phosphorylation of STAT1 and total STAT1 in DMSO- and PA-treated BMDMs under LPS stimulation. f Semi-quantification of (e). g Immunofluorescence staining of p-STAT1 in DMSO- and PA-treated BMDMs under LPS stimulation. h Semi-quantification of (g). i RT-PCR of Stat1 in expression in DMSO- and PA-treated BMDMs under LPS stimulation for 15 min. Scale bar, 50 μm. j Schematic illustration of the pharmacological mechanism of PA. Data are presented as mean ± SD. *p < 0.05, **p < 0.01 and ****p < 0.0001, n = 3.