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. 2023 Mar 10;9:90. doi: 10.1038/s41420-023-01382-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A-E” Single panes of the embryonic CNS; ventral view. Bar, 20 µm. A-B” Control embryos at stage 13 (A-A”) and stage 16 (B-B”) and (C-C”) embryos at stage 16 expressing htl RNAi in glia (gcmGal4/+;repoGal4,cytGFP/UAShtlRNAi) stained with anti-Htl (red) and anti-REPO (blue) antibodies. D-D” Stage 16 control embryos and E-E” embryos expressing htl RNAi in glia (gcmGal4/+;repoGal4,cytGFP/UAShtlRNAi) stained with anti-GFP (green) and anti-Dcp-1 (red) antibodies. F Mean total volume of apoptotic particles within stacks of the CNS and G percentages of apoptotic cell volume that is not engulfed by glia ± SEM, n = 9 (control), n = 6 (htl RNAi). Statistical significance was analyzed employing one-way ANOVA (F) and Student’s t test (G), ****p < 0.0001, ***p < 0.001, *p < 0.05, n.s. (non-significant) p > 0.05. Note the significant increase in volume of apoptotic cells and higher percentage of non-engulfed particles in embryos expressing htl RNAi in glia.