Fig. 8.

Inhibitory effects of type I and III IFNs on CCHFV infection. A–P HEK293 ​cells were pretreated with the indicated concentrations of IFNλ1 (A–H) or IFNλ3 (I–P), or 100 U/mL IFN-α or 100 ​ng/mL IFN-β for 24 ​h, followed by CCHFV infection and RT-qPCR detection at 24 hpi. CCHFV RNA levels (A–C and I–K) and representative ISGs in type III IFN-treated cells (D–H and L–P) were analyzed. (Q and R) Anti-CCHFV activities of type III IFNs in Huh7 cells. Huh7 cells were pretreated with IFNλ1 and IFNλ3 (10 ​ng/mL) for 24 ​h, followed by CCHFV infection and RT-qPCR analyses of the viral RNA (Q) or ISG mRNA (R) levels as above. Data are shown as means ​± ​SD, n ​≥ ​3. ∗∗P ​< ​0.01; ∗∗∗P ​< ​0.001.