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. 2023 Mar 10;14:1323. doi: 10.1038/s41467-023-37094-8

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Immunoblots of ATF4, PHGDH, PKM2, SIRT1 and H3pT11 in the hearts of young mice (6 Months) and aged mice (20 Months). b Representative immunohistochemical staining of PHGDH, PKM2, H3pT11 and SIRT1 in the hearts of young mice (6 Months) and aged mice (20 Months). c Analysis of the relative serine concentrations in the hearts of young mice (6 Months) and aged mice (20 Months). d Immunoblots of PKM2 K305ac and PKM2 K433ac in the hearts of young mice (6 Months) and aged mice (20 Months). e RT-qPCR analysis of ATF4, PHGDH, PKM2 and SIRT1 transcription in the hearts of young mice (6 Months) and aged mice (20 Months). f 18-month-old C57BL/6 mice were injected with AAV that vascular endothelium (VE)-specific expression of PHGDH and PKM2 via tail vein. Four weeks later, protein expression was analyzed and the associated ageing phenotype was examined. g Immunoblots of PKM2, PHGDH, SIRT1, p21 and H3pT11 in the aorta of AAV-Ctrl, AAV-PHGDH and AAV-PKM2 mice. Analysis of body weight (h) and heart weight (i) in AAV-Ctrl, AAV-PHGDH and AAV-PKM2 mice. j Concentration-response curves to acetylcholine obtained in aortic rings from AAV-Ctrl, AAV-PHGDH and AAV-PKM2 mice (4 mice per group). k, l Analysis of echocardiographic parameters, including left ventricular ejection fraction (LVEF), and left ventricular fractional shortening (LVFS) in AAV-Ctrl, AAV-PHGDH and AAV-PKM2 mice. m Total distance and time achieved by AAV-Ctrl, AAV-PHGDH and AAV-PKM2 mice in a treadmill running performance test. For a, c-e, g, j, data represent means ± SE; n = 3 independent experiments. For h, i, k–m, data represent means ± SE, the number of total mice, male mice and female mice were indicated in the graphs; centre lines denote medians, box limits denote 25th and 75th percentiles and whiskers denote maximum and minimum values. Two-sided t-tests were used for statistical analysis.