Table 1.
qPCR Primer Sequences
| Primer | Forward Sequence (5’-3’) | Reverse Sequence (5’-3’) | Primer Efficiency (%) |
|---|---|---|---|
| Actin | CTAGGCACCAGGGTGTGAT | TGCCAGATCTTCTCCATGTC | 120 |
| Axin2 | GTGCCGACCTCAAGTGCAA | GGTGGCCCGAAGAGTTTTG | 110 |
| Cldn5 | GCTCTCAGAGTCCGTTGACC | ATCTAGTGCCCCCAGGATCT | 95.17 |
| Dll4 | AGGCTCCTGCCTTATACCTCT | AGGCTCCTGCCTTATACCTCT | 109 |
| Gfap | CACCTACAGGAAATTGCTGGAGG | CCACGATGTTCCTCTTGAGGTG | 111 |
| Lef1 | AGGGCGACTTAGCCGACAT | GGGCTTGTCTGACCACCTCAT | 130 |
| Hes1 | TCAACACGACACCGGACAAAC | ATGCCGGGAGCTATCTTTCTT | 108 |
| Hey1 | CCGACGAGACCGAATCAATAA | TCAGGTGATCCACAGTCATCT | 113 |
| Map2 | GCTGTAGCAGTCCTGAAAGGTG | CTTCCTCCACTGTGGCTGTTTG | 104 |
| Notch1 | GATGGCCTCAATGGGTACAAG | TCGTTGTTGTTGATGTCACAG | 116 |
| Sox17 | GGCCGATGAACGCCTTTAT | AGCTCTGCGTTGTGCAGATCT | 104 |
| Tie2 | ATCAAGCAACCCAGCCTTTT | ATGCCTGAGCCATTCTCCTT | 113 |
| Cdh5 | CAACTTCACCCTCATAAACAACCAT | ACTTGGCATGCTCCCGATT | 106 |
| Vegfr2 | GCCCTGCTGTGGTCTCACTAC | CAAAGCATTGCCCATTCG | 117 |
| Vegfr3 | CCTGAAGAAGATCGCTGTTC | GAGAGCTGGTTCCTGGAGAT | 102 |
The listed qPCR primers are listed above with the forward and reverse sequences (5’-3’) and primer efficiency.