Fig. 1. Biochemical characterization and cryo-EM structure of NDV L-P complex.
a Elution profile of the purified NDV L-P complex on a Superose 6 Increase size-exclusion column. SDS-PAGE was performed to assess the quality of L-P complex. b Shown the RNA products generated from RNA synthesis reactions. c A representative negative staining micrograph of NDV L-P complex. d A representative cryo-EM micrograph of NDV L-P complex (out of 7134 micrographs). e The final cryo-EM density map of NDV Lc-P complex. f The final cryo-EM density map of NDV Lf-P complex. Source data are provided as a Source Data file. Sample preparation-related experiments including protein purification and enzymatic assays were reproduced at least three times independently.