Fig. 9.

Differential gene expression between ACM and ASY hiPSC-CM in adipogenic condition. A) Heatmap of relative expression (compared to the average of the gene in all samples) of genes found to be significantly differentially expressed between the compared samples (ACM vs ASY). Two replicates were considered for the analysis, excluded for G and E cell lines (see Methods section). B) Volcano plot representing the differential expression results with the log2 differential expression of each gene on the x-axis against its significance expressed as the -log10 adjusted p-value. The blue shaded area defines the used significance criteria. Genes highlighted in red were also found significantly differentially expressed between ACM samples of male and female study participants and thus most likely represent sex-based differential expression. These genes were then excluded in the final comparison analysis between ACM and ASY hiPSC-CM. C) Selected genes by function (also indicated in blue in the volcano plot).